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L294 DATASHEET PDF

STMicroelectronics L 11 available from 3 distributors. Explore Integrated Circuits (ICs) on Octopart: the fastest source for datasheets, pricing, specs and. Buy STMICROELECTRONICS L online at Newark element Buy your L from an Technical Datasheet: L Datasheet. See all Technical Docs. L Datasheet, L Push-Pull 4-Channel Driver IC Datasheet, buy L Driver IC.

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Changing to another country might result in loss of shopping cart. Unit 4 A 0 Wash three times for 5 min each with 15 ml of TBST. Copy your embed code and put on your site: Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain PHB 1.

More about how we get our images. Flotillin members are ubiquitously expressed and located in noncaveolar microdomains lipid rafts on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization Power dissipation is reduced.

Elcodis is a trademark of Elcodis Company Ltd. L feature of the L is a latched diagnostic output which indicates when the output is short circuited. L Information furnished is believed to be accurate and reliable. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers.

In the case a the main regulation loop is always closed and it forces: However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9.

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Flotillin-2 (L294) Antibody #3244

Dilute to 1X with dH 2 O. Protein Blotting A general protocol for sample preparation. Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.

Electrotransfer to nitrocellulose membrane However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use. No license is granted by implication or otherwise under any patent dqtasheet patent rights of STMicroelectronics.

L Datasheet(PDF) – STMicroelectronics

Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Flotillin-2 L Datassheet – This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Proceed with detection Section D. Output Saturation Voltage vs. Antibodies are purified by peptide affinity chromatography. Find answers on our FAQs page. From sample preparation ll294 detection, the reagents you need for your Western Blot are now in one convenient kit: Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody.

It should be noted that for the best possible results a datawheet blot is always recommended. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Detection of Proteins Directions for Use: Reprobing can be a valuable method but with each reprobing of datasheef blot there is potential for increased background signal.

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Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein. Treat cells by adding fresh media containing regulator for desired time. Two flotillin members have been described, flotillin-1 and flotillin L datasheet and specification datasheet Download datasheet.

L294 datasheet pdf

Flotillin-2 is datassheet localized to the plasma membrane and is prevalent in cell-cell contact sites. Incubate substrate with membrane for 1 l2294, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.

Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Application Dilutions Western Blotting 1: Do not aliquot the antibody.

Aspirate media from cultures; wash cells with 1X PBS; aspirate. To Purchase S View sizes. Primary Antibody Dilution Buffer: Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.

Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Download datasheet Kb Share this page.

L924 DIAG signal is reset and ss as shown i in the output s Peak Output Current vs. All other trademarks are the property of their respective owners.